Plasmid Constructs for Overproduction of Chymosin in E. coli

overproduction bacteriorhodopsin in e. coli as pharmacological targets

plasmid for overproduction of the enzyme . ( fre ) of Escherichia coli and construction of a

Cloning and In Vitro-Transcription of Chymosin Gene in E. coli

Chymosin, commonly known as rennin, is the main milk-coagulating enzyme available in rennet. RNA was extracted from the abomasum of a suckling calf water buffalo and was subjected to RT-PCR using degenerate primers to amplify 850bp of the chymosin gene. The sequence was aligned with 19 different mammals' chymosin genes. The sequence revealed that there is a similarity to them ranging from 64% t...

Design of plasmid DNA constructs for vaccines.

For more than three decades, plasmids have been widely used in the biotechnology arena. Historically, they have been most often employed for the expression of heterologous proteins in a variety of microorganisms. More recently, plasmids have been used as vectors for the delivery of antigen encoding genes in order to elicit immune responses in higher order animals. In this chapter, we discuss me...

The Increase in Protein and Plasmid Yields of E. coli with Optimized Concentration of Ampicillin as Selection Marker

Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...